Single radial diffusion procedure manual

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Aim: To study the immunodiffusion technique by Single Radial Immunodiffusion. Introduction: Single Radial Immunodiffusion, also known Single radial immunodiffusion is used extensively for the quantitative estimation of antigens (1). In this method, the antigen-antibody precipitation is Double diffusion in two dimensions is a simple procedure invented by the Swedish scientist, Ouchterlony. Antigen and antibody solutions are placed in separate The method is based on the precipitation in agarose gel of antigen-antibody complexes, and this does not occur with monoclonal antibodies as more than one